Co-expression and Immunity of Legionella pneumophila mip Gene and Immunoadjuvant ctxB Gene
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چکیده
منابع مشابه
Legionella pneumophila mip gene potentiates intracellular infection of protozoa and human macrophages.
Legionella pneumophila is an intracellular parasite of freshwater protozoa and human macrophages. Recent studies determined that the macrophage infectivity potentiator (Mip) surface protein, a prokaryotic homolog of the FK506-binding proteins, is required for optimal infection of macrophages. To determine whether Mip is also involved in L. pneumophila infection of protozoa, we examined the abil...
متن کاملInnate Immunity to Legionella Pneumophila
Innate immune cells, such as macrophages, are highly adapted to rapidly recognize infections by distinct pathogens, including viruses, bacteria, fungi, and protozoa. This recognition is mediated by pattern recognition receptors (PRRs), which are found in host cell surface membranes and the host cell cytoplasm. PRRs include protein families such as the toll-like receptors, nod-like receptors, RI...
متن کاملفراوانی لژیونلا پنوموفیلا در شیر آب سرد و گرم و مخزن آب انکوباتورهای بخش نوزادان بیمارستانهای گیلان
Background and purpose: Nosocomial outbreaks of legionnaires’ diseases are usually related to contamination of water sources. This survey investigated the frequency of mip gene in cold and warm water taps and water containers of infant incubators containing legionella pneumophila in hospitals of Guilan province, Iran. Materials and methods: This cross-sectional study used 140 samples. They wer...
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The nucleotide sequence of the L pneumophila recA gene was determined. The coding region was 1044 nucleotides (348 codons), specifying a 37,934 Da protein. Preceding the recA gene was a tandem set of transcription regulatory sequences and putative LexA binding sites. When expressed in E. coli, the cloned recA gene yield two proteins with molecular weights of approximately 38,000 and 35,500 Da. ...
متن کاملIdentification and differentiation of Legionella pneumophila and Legionella spp. with real-time PCR targeting the 16S rRNA gene and species identification by mip sequencing.
Fluorescent resonance energy transfer probes targeting the 16S rRNA gene were constructed for a sensitive and specific real-time PCR for identification and differentiation of Legionella pneumophila from other Legionella spp. For identification of non-L. pneumophila spp. by direct amplicon sequencing, two conventional PCR assays targeting the mip gene were established.
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ژورنال
عنوان ژورنال: Acta Biochimica et Biophysica Sinica
سال: 2005
ISSN: 1672-9145,1745-7270
DOI: 10.1111/j.1745-7270.2005.00029.x